Heterogeneity in the frequency and characteristics of homologous recombination in pneumococcal evolution.

TitleHeterogeneity in the frequency and characteristics of homologous recombination in pneumococcal evolution.
Publication TypeJournal Article
Year of Publication2014
AuthorsMostowy R, Croucher NJ, Hanage WP, Harris SR, Bentley S, Fraser C
JournalPLoS Genet
Volume10
Issue5
Paginatione1004300
Date Published2014 May
ISSN1553-7404
Abstract

The bacterium Streptococcus pneumoniae (pneumococcus) is one of the most important human bacterial pathogens, and a leading cause of morbidity and mortality worldwide. The pneumococcus is also known for undergoing extensive homologous recombination via transformation with exogenous DNA. It has been shown that recombination has a major impact on the evolution of the pathogen, including acquisition of antibiotic resistance and serotype-switching. Nevertheless, the mechanism and the rates of recombination in an epidemiological context remain poorly understood. Here, we proposed several mathematical models to describe the rate and size of recombination in the evolutionary history of two very distinct pneumococcal lineages, PMEN1 and CC180. We found that, in both lineages, the process of homologous recombination was best described by a heterogeneous model of recombination with single, short, frequent replacements, which we call micro-recombinations, and rarer, multi-fragment, saltational replacements, which we call macro-recombinations. Macro-recombination was associated with major phenotypic changes, including serotype-switching events, and thus was a major driver of the diversification of the pathogen. We critically evaluate biological and epidemiological processes that could give rise to the micro-recombination and macro-recombination processes.

DOI10.1371/journal.pgen.1004300
Alternate JournalPLoS Genet.
PubMed ID24786281
PubMed Central IDPMC4006708
Grant ListU54 GM088491 / GM / NIGMS NIH HHS / United States
U54 GM088558 / GM / NIGMS NIH HHS / United States
U54GM088558 / GM / NIGMS NIH HHS / United States
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