Inferring the serotype associated with dengue virus infections on the basis of pre- and postinfection neutralizing antibody titers.

TitleInferring the serotype associated with dengue virus infections on the basis of pre- and postinfection neutralizing antibody titers.
Publication TypeJournal Article
Year of Publication2010
Authorsvan Panhuis WG, Gibbons RV, Endy TP, Rothman AL, Srikiatkhachorn A, Nisalak A, Burke DS, Cummings DAT
JournalJ Infect Dis
Volume202
Issue7
Pagination1002-10
Date Published2010 Oct 1
ISSN1537-6613
KeywordsAdolescent, Antibodies, Neutralizing, Antibodies, Viral, Child, Dengue, Dengue Virus, Humans, Neutralization Tests, Serotyping, Thailand, Viral Plaque Assay
Abstract

BACKGROUND: Currently, the only tests capable of determining the serotype associated with dengue virus (DENV) infection require sampling during the period of acute viremia. No test can accurately detect the serotype associated with past DENV infections. The standard assay for determination of serotype-specific antibody against DENV is the plaque reduction neutralization test (PRNT), although performance of this test continues to be evaluated.METHODS: From a cohort study among schoolchildren in Thailand, PRNT values were determined in serum samples collected before and after infection. A multinomial logistic regression model was used to infer the serotype associated with intercurrent DENV infections. Models were validated based on polymerase chain reaction identification of DENV serotypes.RESULTS: The serotype associated with DENV infection inferred by the model corresponded with polymerase chain reaction in 67.6% of cases, and the kappa statistic was 0.479. A model for 35 cases with primary seroconversion correctly identified the DENV serotypes causing infection in 77.1% of cases, compared with 66.9%, using a model for 169 cases with secondary seroconversion. The best model using only postinfection PRNT values correctly inferred the DENV serotype causing infection in 60.3% of cases.CONCLUSIONS: A statistical model based on both pre- and postinfection PRNT values can be used to infer the serotype associated with DENV infections in prospective studies and vaccine trials.

DOI10.1086/656141
Alternate JournalJ. Infect. Dis.
PubMed ID20738205
PubMed Central IDPMC2943243
Grant List1R01 TW008246-01 / TW / FIC NIH HHS / United States
1U01-GM070708 / GM / NIGMS NIH HHS / United States
P01 AI034533 / AI / NIAID NIH HHS / United States
P01 AI034533 / AI / NIAID NIH HHS / United States
R01 TW008246 / TW / FIC NIH HHS / United States
U01 GM070708 / GM / NIGMS NIH HHS / United States
U54 GM088491 / GM / NIGMS NIH HHS / United States
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